Figure 2: Tau phosphorylation in STZ-injected mice. | Scientific Reports

Figure 2: Tau phosphorylation in STZ-injected mice.

From: Insulin deprivation induces PP2A inhibition and tau hyperphosphorylation in hTau mice, a model of Alzheimer’s disease-like tau pathology

Figure 2

Hippocampal proteins from 7–9 months-old mice were probed with the following antibodies: 1. CP13, 2. AT8, 3. pT205, 4. AT180, 5. PHF1, 6. Tau-1, 7. Total tau and 8. β-actin (loading control). Two lanes from representative immunoblots are displayed for each condition. Dividing lines represent areas where lanes from the same blot were removed and the remaining lanes were spliced together. Quantifications of tau phosphoepitopes were normalized to total tau levels. Total tau quantification was normalized to β-actin. Results are expressed as percentage of control group (CTL). Data are mean ± SD. Asterisks indicate significant differences, with *p < 0.05, **p < 0.01 and ***p < 0.001. Data from AT8, pT205, AT180, PHF1, Tau-1 and total tau following a normal distribution were analyzed with one-way ANOVA of variance followed by a Bonferroni’s post hoc test, while Kruskal-Wallis test followed by a Dunn’s post hoc test was used for CP13 and β-actin analysis since data deviated from a normal distribution.

Back to article page