Figure 1: Mab 5H7 recognizes all 11 EV71 subgenogroups and does not cross-react with coxsackievirus A16.

IFA of Vero cells infected with heterologous EV71 virus strains and coxsackievirus A16 (GenBank U05876). Cytopathic effects were visible at 2 days post-infection of cells fixed and labeled with supernatant of Mab 5H7-secreting hybridoma cells. FITC-conjugated anti-mouse Mab was used to detect viral signals. While all 11 EV71 subgenogroups were recognized by Mab 5H7, no signal was observed for CVA16. Magnification: 100×.