Figure 3: S74L escape mutation of VP3 protein abolishes 5H7 binding. | Scientific Reports

Figure 3: S74L escape mutation of VP3 protein abolishes 5H7 binding.

From: Effective in vivo therapeutic IgG antibody against VP3 of enterovirus 71 with receptor-competing activity

Figure 3

Escape mutants of 5H7 were created by incubating EV71 B4 strain with an excess of Mab. Vero cells were then fixed and labeled with 5H7, followed by FITC-coupled secondary antibody. Cells infected with wild-type viruses served as positive controls. Mab 5H7 staining was abolished in the escape mutant carrying the mutation S74L (second row). 4B12, an antibody against EV71 3CD, was used as positive control for infection. RG: virus generated by reverse genetics; WT: wild-type virus; EM: escape mutant. Magnification: 100×.

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