Figure 7: 5H7 prevents EV71 from binding to SCARB2 receptor. | Scientific Reports

Figure 7: 5H7 prevents EV71 from binding to SCARB2 receptor.

From: Effective in vivo therapeutic IgG antibody against VP3 of enterovirus 71 with receptor-competing activity

Figure 7

(A): A pull-down assay was performed with SCARB2, B4 virus, and different antibodies. Virus binding to beads was detected by Western blot with Mab 57 against VP2. Cropped blots were presented. No virus band was detected in the samples incubated with 5H7. IgG: a non-EV71 IgG antibody served as a negative control. B4 only: a control for Western blot. (B) Competitive ELISA was also performed with SCARB2, B4 virus, and different antibodies. B4 virus was incubated with different antibodies, and added to wells coated with SCARB2. Virus binding to the coated wells was detected by guinea pig polyserum against B4. No virus was detected in the samples incubated with 5H7. IgG: a non-EV71 IgG antibody acted as a negative control.

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