Figure 3: The PTSL vaccine induced IFN-γ+ expression in CD4+ and CD8+ T cells.

PBMCs were isolated from 5 piglets on the 14th dpv, and then stimulated with inactivated S. Choleraesuis antigen in vitro. The cells were stained with appropriate anti-pig antibodies before flow cytometry analyses for IFN-γ production in CD4+/CD3+ T cells (A) and CD8+/CD3+ T cells (B). *p < 0.05.