Figure 4: Stard7 deficiency increases epithelial barrier permeability. | Scientific Reports

Figure 4: Stard7 deficiency increases epithelial barrier permeability.

From: The Phosphatidylcholine Transfer Protein Stard7 is Required for Mitochondrial and Epithelial Cell Homeostasis

Figure 4

(a) The effect of Stard7 deficiency on BEAS-2B cell monolayer integrity was assessed by measuring transepithelial electrical resistance (TEER, left panel). The effect of Stard7 deficiency on the transepithelial permeability was determined by measuring apical to basolateral diffusion of FITC-dextran at the indicated time points (right panel). Data are expressed as mean ± SEM, n = 3; *p < 0.05, ***p < 0.0001, BEAS-2B cells vs. Stard7KDBEAS-2B cells. (b) Expression of the adherens junction protein E-cadherin was assessed by immunofluorescent staining of BEAS-2B cells and Stard7KDBEAS-2B cells cultured in the presence or absence of MitoTEMPO. Scale bars = 50 μM. (c) Apical junction protein expression was assessed in BEAS-2B cells and Stard7KDBEAS-2B cells, cultured in the presence or absence of MitoTEMPO. Equal numbers of cells from each experimental group were aliquoted in duplicate, cultured for 48 h, and 20 μg protein from each cell lysate analyzed by SDS-PAGE/western blotting (left panel). Protein bands were quantitated by densitometry, the data normalized to actin, and mean values expressed as percent of BEAS-2B control cells (right panel).

Back to article page