Figure 6: Impact of Stard7 deficiency on OCR, ATP, and ROS in lung epithelial cells. | Scientific Reports

Figure 6: Impact of Stard7 deficiency on OCR, ATP, and ROS in lung epithelial cells.

From: The Phosphatidylcholine Transfer Protein Stard7 is Required for Mitochondrial and Epithelial Cell Homeostasis

Figure 6

(a) Lung epithelial cells were isolated from 4-month-old Stard7f/f and Stard7epi∆/∆ mice and OCR (pMoles/min) measured under basal conditions and in response to the indicated mitochondrial inhibitors. OCR was lower in lung epithelial cells from Stard7epi∆/∆ mice (mean ± SEM, n = 5). (b) Cellular ATP levels were assessed in isolated epithelial cells. Data are presented as mean ± SEM, n = 3. There was a significant increase of ATP in lung epithelial cells of Stard7epi∆/∆ compared to Stard7f/f mice, *p = 0.0115. (c) Isolated lung epithelial cells were incubated with or without DCFDA and analyzed by flow cytometry. ROS levels were significantly increased in lung epithelial cells of Stard7epi/∆∆ mice compared to Stard7f/f mice, *p = 0.0193. Data is presented as MFI (mean ± SEM, n = 3).

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