Figure 3: Changes of the abundance of ATP synthase subunits after the loss of VPs in Shigella strains.
From: Role of the virulence plasmid in acid resistance of Shigella flexneri
(A) and (B) Analysis of the AtpA/D protein complex (A) and AtpA (AtpD) protein subunit (B) from wild-type and deletion mutant Shigella strains using blue native-polyacrylamide gel electrophoresis or isoelectric focusing/sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). (C) Detection of AtpD in protein samples from the wild-type and deletion mutant Shigella strains using western blot analysis. The samples were separated by 12.5% SDS-PAGE, and immunoblotted with anti-AtpD.
