Figure 1: Inverse correlation of SENP7S and SENP7L isoforms in NME, DCIS, and multiple BCa subtypes.
From: Novel SUMO-Protease SENP7S Regulates β-catenin Signaling and Mammary Epithelial Cell Transformation
(A) Schematic illustrating SENP7S and SENP7L isoforms. Green arrows highlight primers for SENP7S, SENP7L-specific primers are indicated with blue arrows, and purple arrows represent primers that detect both isoforms. (B,C) Real-time PCR analysis of human patient samples in the TissueScan Breast Cancer Survey (Origene) was used to compare all SENPs in NME samples (n = 5, (B) or SENP7L and SENP7S in the indicated BCa subtype (n = 35 (C)). (B) Relative mRNA levels are normalized to the housekeeping gene β-actin and values on the y-axis represent 2−ΔCt times 104. (C) Relative mRNA levels are 2−ΔΔCt values and represents expression at a specific BCa type versus normal patient samples. ANOVA and Tukey’s post-hoc analysis and p-values (GraphPad) were based on raw ΔCt, and not 2ΔΔCt values. (D) SENP7S assessed in normal and carcinoma human tissue. IHC with SENP7S-specific antibody and hematoxylin counterstain was performed on human tissue samples (normal, n = 18 and carcinoma, n = 19). SENP7S-positive (red arrows) versus -negative (black arrows) cells are highlighted. (E) The graph represents the mean±SEM of the percent of SENP7S-positive to total cells in 40X images acquired from normal (n = 6) and carcinoma (n = 7).
