Figure 2: SENP7S is an active SUMO Protease that is located in the cytosol.
From: Novel SUMO-Protease SENP7S Regulates β-catenin Signaling and Mammary Epithelial Cell Transformation
(A–C) MCF7 cells were transfected with either Flag-tagged SENP7S (A) or Flag-tagged SENP7L (B). Immunofluorescence studies were performed with either Flag or SENP7 isoform-specific antibodies; DAPI was used to identify the nucleus. Yellow arrows highlight the cytosol SENP7S. (C) Cells from immunofluorescence images (n = 6 per group) were counted and the subcellular distribution of the SENP7 isoform was evaluated as more cytosolic (C > N), more nuclear (C < N), or equal distribution (C = N). If the image presented a DAPI stain but no antibody fluorescence signal, then the cell was designated as “no detectable signal” (NDS). The graph represents the percent of cells with the indicated subcellular distribution of protein identified with an antibody for Flag (α-Flag-SENP7S or α-Flag-SENP7L), SENP7S (α-SENP7S), or SENP7L (α-SENP7L). (D) siRNA was designed for targeted-knockdown of SENP7S (siSP7S). MCF10-2A cells were treated with siSP7S or siNT control for 48hr and whole-cell lysates (WCL) were harvested and immunoblotted (IB) with the indicated antibodies. Global SUMO2/3-conjugated proteins are highlighted with asterisks. Black arrowheads mark the unmodified SUMO2/3.
