Figure 3: SENP7S regulates SUMOylation and Ubiquitylation of β-catenin.
From: Novel SUMO-Protease SENP7S Regulates β-catenin Signaling and Mammary Epithelial Cell Transformation
(A,B) Endogenous β-catenin was immunoprecipitated from MCF10-2A cells that were treated with the indicated siRNA for 48hr. (A) SUMO2/3-modified β-catenin could be detected with the SUMO2/3 antibody at the anticipated molecular weight; asterisks are placed adjacent to SUMOylated β-catenin. (B) Immunoblots of the immunoprecipitated β-catenin with an anti-ubiquitin antibody revealed loss of the poly-ubiquitin chains with SENP7S-targeted knockdown as highlighted with black arrows. (C) Exogenous SENP7S was transfected concurrently to supplement for siRNA-targeted loss of endogenous SENP7S in MCF10-2A cells. β-catenin was immunoprecipitated and interaction with Axin1 was assessed following SDS-PAGE and immunoblotting with an Axin1-specific antibody.
