Figure 5: Increased chromatin recruitment of SUMOylated β-catenin and induction of β-catenin-regulated genes.
From: Novel SUMO-Protease SENP7S Regulates β-catenin Signaling and Mammary Epithelial Cell Transformation
(A,B) Immunofluorescence was utilized to detect subcellular localization of β-catenin in MCF102-A cells following 48hrs with the indicated siRNA treatment. β-catenin distribution (red) was compared against the nuclear marker DAPI (blue); the white dashed line highlights the DAPI-stained nucleus. White arrows illustrate nuclear enrichment of β-catenin, which is significantly elevated in SENP7S-siRNA treated cells. Data shown as mean ± SEM of three independent experiments. Student’s t-test was used to compare between groups and p < 0.01 was considered significant. (C) Chromatin-bound (CB) β-catenin was isolated from treated cells and subject to immunoblot for detection of in vivo SUMO2/3-modification. SUMO2/3 β-catenin conjugates are identified with asterisks. (D) Whole cell lysates from treated MCF10-2A cells were run on SDS-PAGE and immunoblotted for the expression of the indicated proteins; β-actin served as loading control.
