Table 1 Identity or similarity analysis between amino acids used for sanxan biosynthesis and the proteins responsible for gellan, welan, and diutan, respectively.
Sanxan | Amino acids | Predicted function | % Identity (% Similarity) | Accession no. | ||
---|---|---|---|---|---|---|
Gellan | Welan | Diutan | ||||
Biosynthesis of nucleotide-sugar precursors | ||||||
manB/pgmG | 460 | Phosphoglucomutase/phosphomannomutase | 80.3 (87.7) | 80.1 (87.2) | — | AHE56248.1 |
manA | 277 | Mannose-6-phosphate isomerase | 71.7 (80.4) | 71.5 (80.4) | — | AHE57091.1 |
manC | 342 | Mannose-1-phosphate guanylyltransferase | 54.8 (66.7) | 63.2 (76.8) | — | AHE56266.1 |
ugpG | 288 | Glucose- 1-phosphate uridylyltransferase | 76.2 (86.1) | 74.8 (86.9) | — | AHE52897.1 |
ugdG | 454 | UDP-glucose-6-dehydrogenase | 70.8 (80.4) | 70.5 (80.4) | — | AHE52667.1 |
rmlA | 288 | Glucose-1-phosphate thymidylyltransferase | 60.3 (77.4) | 60.6 (76.4) | 61.6 (77.7) | AHE57212.1 |
rmlB | 356 | dTDP-glucose 4,6-dehydratase | 63.1 (74.3) | 63.4 (74.6) | 63.1 (74.3) | AHE57214.1 |
rmlC | 186 | dTDP-4-dehydrorhamnose 3,5-epimerase | 48.2 (62.6) | 49.2 (62.6) | 48.2 (63.1) | AHE57215.1 |
rmlD | 297 | dTDP-4-dehydrorhamnose reductase | 43.4 (56.2) | 42.8 (54.9) | 42.1 (55.2) | AHE57213.1 |
Assembly of the repeat units | ||||||
ssB | 468 | Glucosyl-isoprenylphosphate transferase | 41.6 (57.4) | 43.4 (69.6) | 44.1 (60.1) | AHE57216.1 |
ssQ | 327 | Rhamnosyl transferase | 22.6 (34.7) | 12.5 (17.1) | 21.1 (36.6) | AHE56442.1 |
ssH | 428 | Glycosyl transferase | N | N | — | AHE52671.1 |
ssT | 289 | Glycosyl transferase | N | N | — | AHE56448.1 |
ssX | 652 | Acyltransferase | N | N | — | AHE56441.1 |
ssY | 352 | Acyltransferase | N | N | — | AHE56447.1 |
ssZ | 372 | Acyltransferase | N | N | — | AHE57208.1 |
ssI | 521 | Alginate O-acetylation protein | N | N | — | AHE57204.1 |
Polymerization and export of the repeat units | ||||||
ssC-N | 1-489/751 | Export protein (chain length determinant) | 15.6 (29.9) | 17.6 (32.3) | 16.6 (30.9) | AHE52342.1 |
ssC-C | 490-751/751 | Export protein (tyrosine kinase) | 20.1 (34.1) | 22.8 (36.9) | 22.1 (35.9) | AHE52342.1 |
ssD | 199 | Polysaccharide export protein | 19.5 (26.9) | 17.7 (27.8) | 17.1 (25.1) | AHE52343.1 |
ssG | 459 | Polysaccharide polymerase | 17.5 (27.3) | 21.9 (34.7) | 20.2 (33.6) | AHE52344.1 |
ssS | 482 | Polysaccharide biosynthesis protein flippase | 14.7 (29.0) | 18.7 (33.1) | 16.7 (28.5) | AHE57210.1 |