Table 1 Identity or similarity analysis between amino acids used for sanxan biosynthesis and the proteins responsible for gellan, welan, and diutan, respectively.

From: The evolutionary life cycle of the polysaccharide biosynthetic gene cluster based on the Sphingomonadaceae

Sanxan

Amino acids

Predicted function

% Identity (% Similarity)

Accession no.

Gellan

Welan

Diutan

Biosynthesis of nucleotide-sugar precursors

manB/pgmG

460

Phosphoglucomutase/phosphomannomutase

80.3 (87.7)

80.1 (87.2)

AHE56248.1

manA

277

Mannose-6-phosphate isomerase

71.7 (80.4)

71.5 (80.4)

AHE57091.1

manC

342

Mannose-1-phosphate guanylyltransferase

54.8 (66.7)

63.2 (76.8)

AHE56266.1

ugpG

288

Glucose- 1-phosphate uridylyltransferase

76.2 (86.1)

74.8 (86.9)

AHE52897.1

ugdG

454

UDP-glucose-6-dehydrogenase

70.8 (80.4)

70.5 (80.4)

AHE52667.1

rmlA

288

Glucose-1-phosphate thymidylyltransferase

60.3 (77.4)

60.6 (76.4)

61.6 (77.7)

AHE57212.1

rmlB

356

dTDP-glucose 4,6-dehydratase

63.1 (74.3)

63.4 (74.6)

63.1 (74.3)

AHE57214.1

rmlC

186

dTDP-4-dehydrorhamnose 3,5-epimerase

48.2 (62.6)

49.2 (62.6)

48.2 (63.1)

AHE57215.1

rmlD

297

dTDP-4-dehydrorhamnose reductase

43.4 (56.2)

42.8 (54.9)

42.1 (55.2)

AHE57213.1

Assembly of the repeat units

ssB

468

Glucosyl-isoprenylphosphate transferase

41.6 (57.4)

43.4 (69.6)

44.1 (60.1)

AHE57216.1

ssQ

327

Rhamnosyl transferase

22.6 (34.7)

12.5 (17.1)

21.1 (36.6)

AHE56442.1

ssH

428

Glycosyl transferase

N

N

AHE52671.1

ssT

289

Glycosyl transferase

N

N

AHE56448.1

ssX

652

Acyltransferase

N

N

AHE56441.1

ssY

352

Acyltransferase

N

N

AHE56447.1

ssZ

372

Acyltransferase

N

N

AHE57208.1

ssI

521

Alginate O-acetylation protein

N

N

AHE57204.1

Polymerization and export of the repeat units

ssC-N

1-489/751

Export protein (chain length determinant)

15.6 (29.9)

17.6 (32.3)

16.6 (30.9)

AHE52342.1

ssC-C

490-751/751

Export protein (tyrosine kinase)

20.1 (34.1)

22.8 (36.9)

22.1 (35.9)

AHE52342.1

ssD

199

Polysaccharide export protein

19.5 (26.9)

17.7 (27.8)

17.1 (25.1)

AHE52343.1

ssG

459

Polysaccharide polymerase

17.5 (27.3)

21.9 (34.7)

20.2 (33.6)

AHE52344.1

ssS

482

Polysaccharide biosynthesis protein flippase

14.7 (29.0)

18.7 (33.1)

16.7 (28.5)

AHE57210.1

  1. N: not detected; “—” there is no corresponding genome database.