Figure 4: Validation of candidate biomarkers by western blotting.

(A) Image view of the differentially accumulated spot where the protein was detected.(B) Densitometry analysis of WB. The graph shows the log-standardized abundance of the bands detected by WB against 14-3-3, ZAG, ApoH and OTOP3 proteins through the groups.(C) Serum levels of the proteins of interest examined by WB in the pooled serum samples for each group. The error bars indicate standard error of measurements performed at least in triplicate. Asterisks show significant differences at p < 0.05. Full-length blots are presented in Supplementary Fig. S5.