Figure 2: In vitro virulence factor production is decreased in only two of the five C. neoformans sirtuin mutants. | Scientific Reports

Figure 2: In vitro virulence factor production is decreased in only two of the five C. neoformans sirtuin mutants.

From: Sirtuins in the phylum Basidiomycota: A role in virulence in Cryptococcus neoformans

Figure 2

Spot dilution assays on L-DOPA, Christensen’s, and egg yolk agar were used to determine the level of melanin, urease, and phospholipase B production, respectively. Melanized strains appear brown-black, while urease and phospholipase B production appear as halos surrounding the colony. Plates were grown at both 30 and 37 °C.

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