Figure 7: Silencing fantom3_F730004F19 attenuated the inflammatory response. | Scientific Reports

Figure 7: Silencing fantom3_F730004F19 attenuated the inflammatory response.

From: High-Throughput Sequencing and Co-Expression Network Analysis of lncRNAs and mRNAs in Early Brain Injury Following Experimental Subarachnoid Haemorrhage

Figure 7

(A–F) Inhibition of fantom3_F730004F19 reduced the expression of CD14 and TLR4 in BV-2 cells following LPS treatment. Protein expression was shown to be completely depleted by both western blot analysis (A–C) and immunofluorescence staining (D–F) (full-length blots/gels are presented in Supplementary Figure S5). Although the inhibitory effect was not obvious in the normal condition, silencing fantom3_F730004F19 significantly reduced the expression of CD14 and TLR4 protein levels after LPS treatment (#P < 0.05 and ##P < 0.01 versus the NC LPS-treated group). (G–I) Inhibition of fantom3_F730004F19 attenuated inflammatory cytokines release. Silence fantom3_F730004F19 significantly reduced the release of IL-1β (G), IL-6 (H) and TNF-α (I) after LPS treatment (#P < 0.05 and ##P < 0.01 versus the NC LPS-treated group). N: normal condition group; LPS: lipopolysaccharide treated; Blank: non-transfected cells; NC: negative control lentivirus transfected cells; KD: the lncRNA fantom3_F730004F19 lentivirus transfected cells.

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