Figure 4: Currents and NLC associated with prestin are separable.
From: Current carried by the Slc26 family member prestin does not flow through the transporter pathway
(A,B). Shown are NLC (A) and currents (B) in a single cell perfused with extracellular Cl−, OCN− and SCN−. SCN− increases current significantly and affects gating charge, while OCN− causes a slight increase in current while causing a hyperpolarizing shift in Vh. (C,D) Extracellular salicylate (20 mM) has no effect on currents (C) in the presence of extracellular SCN−, while reducing NLC (D). 1 mM DIDS does not affect NLC or SCN− current. Shown are average tracings from three cells (+/−SEM). (E,F) The effects of fluid-jet pressure on the size of the current are dependent on the conducting anion. With Cl− in the bath solution there is an increase in the size of the current with exposure of the cell to a fluid jet (E). In contrast, in the presence of extracellular SCN−, the size of the current decreases with exposure to a fluid jet (F). Shown are average tracings from 10 cells (+/−SEM).
