Table 1 PglK ATPase activity and Nbs effect.

From: Structural basis of inhibition of lipid-linked oligosaccharide flippase PglK by a conformational nanobody

 

LMNG

Proteoliposomes

nmolPi/mg protein x min

nmolPi/mg protein x min

−LLO

+LLO

−LLO

+LLO

PglK-WT

58.2 +/− 2.1

104.8 +/− 8.3

58.3 +/− 4.5

118.9 +/− 15.4

PglK-WT + Nb80

57.1 +/− 3.4

97.6 +/− 9.6

62.3 +/− 5.1

104.5 +/− 12.3

PglK-WT + Nb87

4.1 +/− 1.7

6.3 +/− 3.2

11.3 +/− 3.5

21.4 +/− 9.2

PglK-WT + NbPglB

62.1 +/− 5.1

125.8 +/− 15.3

61.0 +/− 5.3

117.3 +/− 13.8

PglK-WT (pre-ATP) + Nb87

6.0 +/− 0.4

9.5 +/− 0.5

14.4 +/− 1.3

28.6 +/− 3.4

Back to article page