Figure 3: Influence of glucose supplementation on mild MPP+ exposure-induced autophagosome accumulation.

(a) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for up to 48 h; LC3-II expression levels at several time points were detected by western blotting. (b) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for 48 h with or without 5.5 mM glucose for the last 12 h; LC3-II expression was detected by western blotting. (c) SH-SY5Y cells were exposed to 10 and 200 μM MPP+ for 48 h with or without 5.5 mM glucose for the last 12 h; p62 expression was detected by western blotting. Data are expressed as means ± S.D. from at least three independent experiments. *p < 0.05, **p < 0.01.