Figure 5: Visual characterization of the aggregation behaviour of AMTN and ODAM.

(a–c) Transmission electron microscopy (TEM) of negative stain and (d–f) atomic force microscopy preparations of purified AMTN and ODAM demonstrate the propensity of the proteins to self-interact into globular complexes. Mixing the two proteins together generates larger complexes suggesting heterologous interactions. (Insets in a,b) Observation of the complexes under native conditions by cryo-TEM confirms that they do not result from preparation artefacts.