Figure 1: TGF-β3 treatment enhances the expression of chondrogenic associated marker genes, enhances chondrogenic associated histological staining and enhances the expression of chondrogenic associated miR-140-3p and miR-140-5p and decreases the expression of miR-146b in human bone marrow derived SSCs. | Scientific Reports

Figure 1: TGF-β3 treatment enhances the expression of chondrogenic associated marker genes, enhances chondrogenic associated histological staining and enhances the expression of chondrogenic associated miR-140-3p and miR-140-5p and decreases the expression of miR-146b in human bone marrow derived SSCs.

From: MiR-146b is down-regulated during the chondrogenic differentiation of human bone marrow derived skeletal stem cells and up-regulated in osteoarthritis

Figure 1

Data is presented as the median and interquartile quartile range of the fold change in SOX9 mRNA (A), AGCAN mRNA (B) COL2A1 mRNA (C) COL9A1 mRNA (D), miR-140-3p (H), miR-140-5p (I) and miR-146b (J) expression in human bone marrow derived SSCs cultured in the presence of TGF-β3 for 21 days relative to untreated control bone marrow derived SSCs cultured in the absence of TGF-β3 for 21 days. n = 6, *p < 0.05, Wilcoxon signed rank test. Safranin O stain (E), Alcian blue stain (F) and type II collagen immuno-staining (G) and haematoxylin counterstain in human bone marrow derived SSC micromass cultures after 21 days in the presence and absence of TGF-β3 (inset). Scale bar = 200 μm (E–G) 100 μm (inset E–G).

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