Figure 4: Dysregulated mRNA, miR-146a and miR-146b expression is observed in OA articular chondrocytes compared to control chondrocytes. | Scientific Reports

Figure 4: Dysregulated mRNA, miR-146a and miR-146b expression is observed in OA articular chondrocytes compared to control chondrocytes.

From: MiR-146b is down-regulated during the chondrogenic differentiation of human bone marrow derived skeletal stem cells and up-regulated in osteoarthritis

Figure 4

Data presented as the median and interquartile range of the fold change in MMP13 mRNA (A), COL2A1 mRNA (B) and AGCAN mRNA (C), miR-146a (D) and miR-146b (E) expression in OA articular chondrocytes relative to control chondrocytes. Values are presented as individual biological replicates n = 11, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Mann-Whitney U test. Supplementary Figure S1. Full length images of the cropped blots presented in main Fig. 3D. Full length images in Figure S1 demonstrate that increased levels of miR-146b in human bone marrow derived SSCs reduces SOX5 expression. Human bone marrow derived SSCs were cultured in the presence of miR-146b mimic and non-targeting miRNA mimic. Human bone marrow derived SSCs were isolated from 6 individual patient samples. β-actin was used as the internal control.

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