Table 1 Primer sequences for genes examined in and corresponding amplicon size.
Gene | Primer Sequences | Amplicon size |
|---|---|---|
Human ACTB | F: 5′ggcatcctcaccctgaagta 3′ R: 5′aggtgtggtgccagattttc 3′ | 82 bp |
Human SOX9 | F: 5′cccttcaacctcccacacta 3′ R: 5′ tggtggtcggtgtagtcgta 3′ | 74 bp |
Human COL2A1 | F: 5′cctggtccccctggtcttgg 3′ R: 5′ catcaaatcctccagccatc 3′ | 58 bp |
Human ACGAN | F: 5′gacggcttccaccagtgt 3′ R: 5′gtctccatagcagccttcc 3′ | 90 bp |
Human COL9A1 | F: 5′cctggtgctcttggtttga 3′ R: 5′ cacgctcccccttttctc 3′ | 58 bp |
Human MMP13 | F: 5′ttaaggagcatggcgacttct 3′ R: 5′ cccaggaggaaaagcatgag 3′ | 71bp |
Human SOX5 | F: 5′tagctagtccttcagccagagtt 3′ R: 5′ccttcatttgccgagcttctt 3′ | 93 bp |
