Figure 2: Expression of Klf5 and Dsp in dental cells during mouse tooth development.

(a–e) in iMDP-3 cells, (f–j) in MD10-F2 cells, the coexpression of Klf5 (green, b and g) with Dsp (red, c and h) was observed in these cells. (k–o) in molar at PN2, (p–t) in molar at PN6, Klf5 (green, l and q) expression was observed in ameloblasts, SI, HERS, odontoblasts and dentin. Expression of Dsp (red, m and r) was also overlapped with that of Klf5. a,f,k and p show bright images. (d,i,n and s) Cells were stained with Hoechst for nuclei. e,j,o and t are merged. Similar to the above description, (a′–t′) in incisors at PN3, coexpression of Klf5 (green, e′–h′) and Dsp (red, i′–l′) was visible in odontoblasts. a′–d′ show bright images. (m′–p′) Cell nuclei were stained with Hoechst. (q′–t′) The images were merged. Bars, 20 μm (a–t), 10 μm (k–t, a′–t′).