Figure 5: Klf5 transactivates Dspp and Dmp1 promoter activity in iMDP-3 cells. | Scientific Reports

Figure 5: Klf5 transactivates Dspp and Dmp1 promoter activity in iMDP-3 cells.

From: Klf5 Mediates Odontoblastic Differentiation through Regulating Dentin-Specific Extracellular Matrix Gene Expression during Mouse Tooth Development

Figure 5

iMDP-3 cells were transfected with different Dspp and Dmp1 promoter constructs with either pcDNA3-Klf5 or pcDNA3 plasmid as control. Reporter activities were measured by a dual-luciferase assay in the presence or absence of pcDNA3-Klf5 co-transfection. (a) Activation of Dspp promoters containing pGL3-5.7 kb, pGL3-2.6 kb, pGL3-1.5 kb, pGL3-1,318 bp and pGL3-591bp was increased 5.8-, 3.0-, 1.8-, 1.9- and 1.1-folds, respectively. (b) Effect of different Klf5 concentrations on Dspp promoter activity containing pGL3-5.7 kb. (c) The transcription activities appeared to increase in a dosage-dependent manner for Dspp promoter-5.7 kb with Klf5 transfection. (d) Activation of Dmp1 promoters containing pGL3-2.6 kb, pGL3-1,656 bp, pGL3-1,187 bp, pGL3-656bp and pGL3-213bp were increased 5.4-, 4.0-, 3.2-, 2.2- and 2.0-folds, respectively. (e) Effect of different Klf5 concentrations on Dmp1 promoter activity containing pGL3-2.6 kb. (f) The transcription activities appeared to increase in a dosage-dependent manner for Dmp1 promoter-2.6 kb with Klf5 transfection. Luciferase (Luc) activity was normalized to the control group. *P < 0.05; **P < 0.01.

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