Figure 3

Mouse behavioral responses to the 5-HT_2A agonist 2,5-dimethoxy-4-iodoamphetamine (DOI) are attenuated by genetic deletion of caveolin-1 (Cav-1). (a) Mouse head twitch responses to the 5-HT_2A agonist DOI are decreased by Cav-1 knockout (KO). Littermate wild-type (WT) and Cav-1 KO mice were injected with 1.25 mg kg⁻¹ DOI and head twitches scored over a 35-min period; DOI-induced head twitches were attenuated in Cav-1 KO mice at all-time points (panel a, mean±s.e.m., n=12 littermate pairs; ^*P<0.05 versus WT mice). (b) Dose responses of DOI on total mouse head twitches over a 35-min period are shown; head twitches were attenuated in Cav-1 KO mice only at higher concentrations (panel b, mean±s.e.m., n=6 to 8 littermate pairs; ^*P<0.05 versus WT mice at each concentration). (c) DOI disruption of sensory motor gaiting in mice is prevented by Cav-1 KO. Littermate WT and Cav-1 KO mice were injected with saline, 1 or 5 mg kg⁻¹ DOI and 10 min later placed into acoustic startle response chambers to assess prepulse inhibition (PPI). DOI significantly decreased (disrupted) PPI at 4 and 8 dB prepulse levels in WT but not Cav-1 KO mice (panel c, mean±s.e.m., n=8 littermate pairs; ^*P<0.05 versus WT saline-treated mice at each prepulse level). NS, nonsignificant.