Figure 1

Time-lapse imaging of pyramidal neuron migration in cortical slices. (a, b) Confocal images showing that TOM+ pyramidal neurons display radially orientated migratory-like morphologies in the E17.5 developing cortex (a) and reach their final destination in superficial cortical layers at E19 (b). Pyramidal neurons were labeled using in utero electroporation of a TOM+-expressing plasmid in the ventricular zone (VZ) at E14.5. (c) Epifluorescent time-lapse images taken from a 12-h time-lapse sequence showing TOM+ neurons (arrowheads) migrating radially toward the pial surface in a E17.5 cortical slice. Superposed color lines represent migratory tracks. (d) Graph showing the migratory distances travelled by TOM+ cells shown in panel c2. (e, f) Graphs showing that the mean migratory speed of TOM+ cells does not significantly change during a 12-h time-lapse sequence in E17.5 TOM+ cells labeled at E14.5 (e) and in P0.5 TOM+ cells labeled at E17.5 (f). White boxed area depicts a higher magnification image. Scale bars: 50 μm.