Figure 3

The 5-HT₆ receptor is expressed in pyramidal neurons during cortical development. (a–c) In situ hybridization showing strong expression of the 5-HT₆ receptor at E17.5 (a, b) and P0.5 (c). (d) Quantitative PCR graph showing no significant fold change in the cortical expression of the 5-HT₆ receptor. (e) Confocal image showing that the 5-HT₆ receptor antibody used in this study specifically recognizes 293 T cells transfected with a 5-HT₆-EGFP-over-expressing plasmid. (f, g) Confocal images showing that TOM+ neurons (f) and GAD65-GFP+ cells (g) express the 5-HT₆ receptor at day in vitro 1 in cortical cultures. TOM+ cells were electroporated at E14.5 in GAD-65-GFP mice, and cortical cultures were prepared at E17.5. (h–j) Composite confocal images showing strong 5-HT₆ receptor immunoreactivity at E17.5 in the cortical plate (i) and in the ventricular zone (VZ)/SVZ (h) and in superficial cortical layers at P0.5 (j). Scale bars: 100 μm for a–c, 10 μm for e–g, 50 μm for h–j. GFP, GAD65-GFP; Hst, Hoechst; aTub, acetylated tubulin.