Figure 2 | Translational Psychiatry

Figure 2

From: Early accumulation of intracellular fibrillar oligomers and late congophilic amyloid angiopathy in mice expressing the Osaka intra-Aβ APP mutation

Figure 2

Accumulation of detergent-insoluble amyloid β (Aβ) at different ages in the three Alzhemier’s disease (AD) mouse models. MesoScale Discovery (MSD) analysis of Aβ38 (black column), Aβ40 (gray column) and Aβ42 (white column) in serially extracted cortical samples (Tris buffer, Tris buffer containing 1% Triton, Tris buffer containing 2% sodium dodecyl sulfate (SDS) and formic acid (FA)). (a) In E22ΔAβ mice most of the Aβ can be detected in the SDS-soluble protein fraction up to the age of 15 months, whereas Tris buffer-soluble, Triton-soluble and FA-soluble Aβ levels are comparatively low. Only at 24 months of age is detergent-insoluble Aβ detected. (b) In contrast, Tg2576 mice show higher soluble Aβ levels in Tris and Triton fractions and a prominent age-dependent Aβ accumulation in the detergent-insoluble FA fraction at 15 months. (c) The 3-month-old arcAβ mice show intermediate Tris- and Triton-soluble Aβ levels (as compared with age-matched E22ΔAβ and Tg2576 mice) and accumulation of Aβ in the FA fraction already at the age of 6 months. n=6–9 per group.

Back to article page