Table 4 qPCR validation of candidate gene expression

Gene	Probe		0-week				8-week				Mixed linear model
	Microarray	qPCR	Micro-array	qPCR			Micro-array	qPCR			P-value	Group hierarchy
			FC	FC _A	FC _B	FC _all	FC	FC _A	FC _B	FC _all
ACTBL2	A_24_P6903	Hs01101944_s1	1.69^a	−1.32	1.52	1.06	1.76^b	−1.03	1.17	1.05	0.025	Responder<non responder
ATP2A2	A_24_P141786	Hs00155939_m1	1.72^a	1.60	1.00	1.36	1.55^a	1.56	1.41	1.50	0.904	No
CELF2	A_23_P115645	Hs00990166_m1	1.54^b	1.05	1.08	1.06	1.48	1.14	1.01	1.09	0.985	No
HIST1H1A	A_23_P70448	Hs00271225_s1	−1.93^b	−1.20	1.71	1.18	−1.76	−1.05	1.24	1.07	0.019	Responder<non responder
HIST1H1E	A_23_P7976	Hs00271195_s1	−1.94^a	−1.22	1.15	−1.05	−1.66^b	1.10	1.16	1.13	0.005	Responder<non responder
HIST1H4E	A_23_P415411	Hs00374346_s1	−2.23^a	−1.18	1.44	1.08	−2.18^a	−1.15	1.14	−1.03	0.010	Responder<non responder
IL1B	A_23_P79518	Hs00174097_m1	3.31^b	4.12^##	−1.22	2.80	3.17	6.56	−1.17	4.28	0.001	Responder>non responder
IRF2	A_23_P125082	Hs01082884_m1	2.00^a	1.25^#	1.10	1.20	1.63^b	1.11	1.03	1.08	0.194	No
NRG1	A_23_P315815	Hs00247624_m1	2.01^b	1.74	1.19	1.52	1.87	1.79	2.10	1.92	0.327	No
POTEKP	A_32_P155776	Hs02598440_g1	1.66^a	−1.16	1.76	1.22	1.61^b	1.01	1.40	1.16	0.011	Responder<non responder
PPT1	A_24_P276628	Hs00165579_m1	2.30^a	1.21^##	−1.09	1.10	2.22^b	1.28^#	1.01	1.17	0.00005	Responder>non responder
SORT1	A_24_P325520	Hs00361760_m1	1.85^b	1.68^#	1.31	1.53	1.47	1.25	1.22	1.23	0.775	No
TNF	A_23_P376488	Hs00174128_m1	1.68	1.54^#	−1.03	1.31	2.61^b	2.81	1.11	2.13	0.00047	Responder>non responder
TPP1	A_24_P38815	Hs00166099_m1	1.55^a	1.04	−1.04^##	1.01	1.51^b	1.04	−1.04	1.01	0.051	Responder>non responder

Abbreviations: FC, fold change; FDR, false discovery rate; PPT1, palmitoyl-protein thioesterase 1; qPCR, quantitative PCR.
^#P<0.05; ^##P<0.01.
The last 2 columns display the P-value and hierarchy of significance in differences between the responder versus non responder groups, using a statistical mixed linear model, taking into account two groups (responder and non responder), the three first visits (0, 2, 8-week), and corrected by age and sex parameters.
^aFDR<1%.
^bFDR<5%.

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