Figure 5

GSK-3β inhibition blocks the effect of IL-1β on TLX expression in differentiated hippocampal neurons. Representative photomicrographs of cells immunocytochemically stained for doublecortin (DCX) (red), βIII-tubulin (red), and glial fibrillary acidic protein (GFAP) (red) with TLX (green) (a and b) or glycogen synthase kinase-3beta (GSK-3β) (red) with TLX (green) (c) in untreated and interleukin-1β (IL-1β)-treated cultures under differentiation conditions for 7 DIV. (b A and B) Arrow indicates cell undergoing mitosis. Cells were counterstained with DAPI (blue) to visualize the nuclei. Scale bar=50 μm. Mean densitometry analysis of TLX protein in DCX⁺ neurons (d), βIII-tubulin⁺ neurons (e) and GFAP⁺ astrocytes (f) in untreated, IL-1β-treated, SB216763-treated and IL-1β+SB216763-treated cultures. Data are expressed as means±s.e.m. *P<0.05, vs. untreated control; ^&&&P<0.001 vs IL-1β treatment alone (two-way analysis of variance (ANOVA) with Bonferroni post hoc test; n=3).