Figure 4
From: Sustained remission from depressive-like behavior depends on hippocampal neurogenesis
Exposure to unpredictable chronic mild stress (uCMS) causes long-term neuromorphological alterations in newborn granule neurons. (a) Structural changes in the dendritic arborization of preexisting granule neurons in the hippocampal dentate gyrus (DG), analyzed with the Golgi-Cox impregnation method, induced by uCMS exposure are reverted at long-term (4 weeks after uCMS exposure). (b–d) uCMS exposure does not have long-term impact on the dendritic arborization (b, c), neither in the global spine density of newborn neurons in the hippocampal DG (d), analyzed with the Immuno-Golgi method (co-labeling of BrdU and Golgi-Cox staining). (e, f) Newborn granule neurons of rats exposed to uCMS 4 weeks before, present increased percentage of thin spines; these alterations are attenuated in the animal groups treated with both antidepressants (e, f). ⋄Denotes the effect of uCMS; ⋄P<0.05; n=10–15 per group.
