Table 2 APOBEC mRNA expressions

From: Upregulation of TET1 and downregulation of APOBEC3A and APOBEC3C in the parietal cortex of psychotic patients

Variable (mRNA)

Relative APOBEC mRNA expression a

Overall ANOVA

Multiple comparison

 

1 CTR

2 DEP

3 PSY

F

d.f.

P-value

DEP vs

PSY vs

       

1

3

1

2

APOBEC3A

1.0±0.23

0.15±0.06

0.31±0.12

15.38

2

0.0001

0.001

—

0.0001

—

APOBEC3B

1.0±0.05

0.74±0.07

0.42±0.02

1.26

2

NS

—

—

—

—

APOBEC3C

1.0±0.02

0.58±0.04

0.50±0.03

5.718

2

0.01

—

—

0.01

—

APOBEC3D

1.0±0.01

0.69±0.01

1.02±0.01

0.782

2

NS

—

—

—

—

APOBEC3F

1.0±0.12

0.80±0.08

0.88±0.07

0.173

2

NS

—

—

—

—

APOBEC3G

1.0±0.13

1.08±0.11

0.97±0.04

0.081

2

NS

—

—

—

—

APOBEC3H

1.0±0.05

0.86±0.04

0.68±0.03

0.561

2

NS

—

—

—

—

APOBEC4

1.0±0.10

1.02±0.17

1.09±0.11

0.198

2

NS

—

—

—

—

  1. Abbreviations: AID, activation-induced deaminase; APOBEC, apolipoprotein B mRNA-editing enzyme; CTR, nonpsychiatric; DEP, depressed; d.f., degree of freedom; IPL, inferior parietal lobule; NS, nonsignificant; PSY, psychotic.
  2. Note that AID, APOBEC1 and APOBEC2 were not detected in the IPL of our cohort, but were considerably abundant in NT2 cell line (human neuronal progenitor cell), indicating that their expressions are extremely low in terminally differentiated neurons or glial cells. The relative basic expression levels (2-ΔCt × 10−3 vs β-actin) of APOBECs measured in the IPL of the CTR group are: 0.71±0.23 (APOBEC3A); 0.11±0.05 (APOBEC3B); 2.5±0.5 (APOBEC3C); 0.11±0.04 (APOBEC3D); 0.42±0.97 (APOBEC3F); 0.55±0.16 (APOBEC3G); 0.15±0.07 (APOBEC3H) and 1.6±0.36 (APOBEC4), indicating that the APOBEC3 subgroup genes are expressed heterogeneously in the IPL.
  3. aThe expression of APOBEC3A was decreased both in the PSY and the DEP groups compared with the CTR group, while the expression of APOBEC3C mRNA was decreased only in the PSY group. Each value represents the mean±s.e.m. of relative quantity (2−ΔΔCt) of APOBECs vs β-actin.
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