Figure 1

Generation of mice lacking Lepr in forebrain neurons. (a, left panel) In situ hybridization showing the long form of Lepr (Lepr-b) mRNA expression. (a, right panel) Fluorescence immunohistochemistry of β-galactosidase indicating that Cre activity under the control of Emx1 promoter was restricted to the hippocampal and cortical neurons. (b) Reverse transcription-PCR analysis of gene expression of Exon 17 of the leptin receptor in the hippocampus, cortex and hypothalamus. (c) Real-time quantitative PCR analysis of gene expression of Exon 17 of Lepr in the hippocampus, cortex and hypothalamus. ^*P<0.05 compared with fWT controls. (d) Body weight gain of Lepr cKO mice and fWT littermate controls in males and females from 4 to 12 weeks of age. Male fWT, n=19; male Lepr cKO, n=19; female fWT, n=17; female Lepr cKO, n=20. (e) Body composition in both male and female mice; n=8 per group. (f) Plasma concentrations of leptin; n=5 per group. (g) Plasma concentrations of insulin; n=5 per group. All data are presented as mean±s.e.m.