Figure 3

(a) Representative examples of changes in 5mC and mRNA expression after a 6-h sleep deprivation (SD) in the mouse cerebral cortex. (b) Correlation between DNA methylation differences measured by 5mC-immunoprecipitation (IP)-arrays and validated by 5mC-IP-quantitative PCRs. (c) Expanded views from the UCSC genome browser at the Dlg4 gene location. The first track shows average methylation probe fold differences (Log2) and the second shows regions significantly differentially methylated. The last track shows exons and introns taken from the mouse NCBI RNA reference sequence collection (RefSeq). #P<0.1, *P<0.05, **P<0.01, ***P<0.001 compared with control (Ctrl).