Figure 4 | Translational Psychiatry

Figure 4

From: The genome-wide landscape of DNA methylation and hydroxymethylation in response to sleep deprivation impacts on synaptic plasticity genes

Figure 4

(a) Heat map of 4000 more significant probes showing an effect of sleep deprivation (SD) on 5hmC in the mouse cerebral cortex. Columns refer to three pools of DNA of three control (Ctrl) and three SD mice (total nine per group). Transcripts were ordered by hierarchical clustering (complete linkage). (b) Differentially 5hmC sites were enriched in exons (P<10E–300, Fisher's Exact Test); TTS/3′-untranslated region (P<10E–300) but negatively enriched in promoters (P<10E–300), introns (P<6.8E–11) and intergenic regions (P<1.6E–43). (c) Correlation between DNA hydroxymethylation differences measured by 5hmC-IP-arrays and 5hmC-IP-quantitative PCRs for 15 different genomic regions. (d) Changes in 5hmC after a 6-h SD for selected targets. (e) Selected changes in 5hmC and mRNA expression after a 6-h SD. #P<0.1, *P<0.05, **P<0.01, ***P<0.001 compared with control (Ctrl).

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