Table 1 Summary of mRNA levels and DNA binding of TET1 (a) and DNMT1 (b) to the RELN and GAD1 promoters in cerebella of ASD vs CON

From: Increased binding of MeCP2 to the GAD1 and RELN promoters may be mediated by an enrichment of 5-hmC in autism spectrum disorder (ASD) cerebellum

 

(a)

ChIP (% of DNA input)

(b)

ChIP (% of DNA input)

 

TET1 relative mRNA

TET1 binding to RELN

TET1 binding to GAD1

DNMT1 relative mRNA

DNMT1 binding to RELN

DNMT1 binding to GAD1

CON

0.021±0.010

0.011±0.021

0.070±0.011

0.76±0.25

0.20±0.010

0.11±0.020

ASD

0.052 ±0.010

0.031±0.064

0.100±0.013

0.60±0.11

0.16±0.030

0.13±0.020

P-value

0.01

0.01

0.046

0.6

0.5

0.6

  1. Abbreviations: ASD, autism spectrum disorder; ChIP, Chromatin ImmunoPrecipitation; CON, control. % of DNA input was calculated as 2(Ct(10% input)−3.32)−Ct(gene−IP) × 100% where IP is the amount of specific gene-immunoprecipitated DNA and input is the amount of the same gene in the starting material. Relative mRNA levels were calculated relative to the amounts of GAPDH mRNA.
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