Figure 4 | Translational Psychiatry

Figure 4

From: Independent and epistatic effects of variants in VPS10-d receptors on Alzheimer disease risk and processing of the amyloid precursor protein (APP)

Figure 4

γ-Secretase activity and nuclear translocation of amyloid precursor protein (APP) assays with sortilin-related VPS10 domain-containing receptor 2 (SORCS2), SORCS3 or SORT1 short hairpin RNAs (shRNAs). Both the APP-GV (GAL4 DNA-binding domain with VP16 transcriptional activator) and APP gene’s C-terminus (AICD)-GV assay were performed in HEK293 cells. The data from SorCS2, SorCS3 or sortilin 1 (SORT1) shRNA was normalized to either APP-GV only or AICD-GV with the scrambled sequence shRNA (shRNA-scrambled), which was included as a negative control. The data are representative for the assays, were performed in ⩾3 experiments in replicates of eight samples per condition (96-well format, s.d. bars are shown, *P<0.05, **P<0.01, ***P<0.001 as compared with APP-GV only (analysis of variance, Bonferroni’s correction).

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