Figure 1
From: Autism-specific maternal autoantibodies recognize critical proteins in developing brain
Prep cell protein fractionation and two-dimensional (2D) gel. (a) Ponceau stained nitrocellulose membrane containing samples from every sixth fraction collected from Prep Cell separation of Rhesus fetal brain protein. (b) Western blots of the membranes in a probed with maternal plasma reactive against the target bands. (c) Duplicate 2D gels were run with target region 30–40 kDa, 39–50 kDa and 60–85 kDa optimized Prep Cell fractions. The left column shows chemiluminescent images of 2D western blot probed with diluted plasma from mothers reactive against each of the antigens. Circles on the gels in the right column represent spots of reactivity between maternal antibodies and cognate antigens on the 2D blots on the left that were used to guide spot picking.
