Figure 1 | Translational Psychiatry

Figure 1

From: Transcripts involved in calcium signaling and telencephalic neuronal fate are altered in induced pluripotent stem cells from bipolar disorder patients

Figure 1

Derivation, characterization and neuronal differentiation of iPSC. Fibroblasts (a) express Te-7 (b; Cy3-secondary antibody, red). Reprogrammed colonies of iPSC form and are passaged to MEFs. They downregulate Te-7 (c; Cy3-secondary antibody), and exhibit nuclear expression of Nanog (d; FITC-secondary antibody). For differentiation, cells are removed from MEF and grown in defined medium as neurospheres (e), then plated to form rosettes (f). Rosettes are picked twice, then differentiated in adherent culture in defined medium forming nestin + (g; FITC-secondary antibody) neural progenitor cells, and with additional time in vitro undergo widespread differentiation to β-III tubulin + neurons (h; Cy3-secondary antibody), with punctate expression of Synapsin 1 (Syn1) along their processes (i, Cy3-secondary antibody). All cells, but a, are stained with Hoechst 23487 (blue) to identify nuclei. Scale bars in a–d,g = 500 mm; e = 2 mm, f,h,i = 200 mm.

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