Figure 3 | Translational Psychiatry

Figure 3

From: Leptin receptor deficiency confers resistance to behavioral effects of fluoxetine and desipramine via separable substrates

Figure 3

Effects of acute fluoxetine treatment on phosphorylation of Akt, GSK3β, ERK1/2 and total protein levels of SERT in the hippocampus, PFC and hypothalamus of db/db mice versus wild-type mice. (a) Phosphorylation of Akt, GSK3β and ERK1/2 was determined 30 min after acute fluoxetine treatment (20 mg kg−1, i.p.). Upper panel showing representative immunoblots of Akt, GSK3β and ERK1/2 in the hippocampus of wild-type (WT) mice and db/db mice. Bottom panel showing quantitative data. (b) Phosphorylation of Akt, GSK3β and ERK1/2 in the PFC of wild-type mice and db/db mice. (c) Phosphorylation of Akt, GSK3β and ERK1/2 in the hypothalamus of wild-type mice. n=3–5 per group. *P<0.05, **P<0.01, ***P<0.001 compared with vehicle-treated wild-type mice. (d) SERT protein levels in the hippocampus and PFC show no genotype difference. n=4 per group. i.p., intraperitoneal; PFC, prefrontal cortex; SERT, serotonin reuptake transporter.

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