Figure 2
Temporal expression pattern and subcellular localization of CDH13. (a) Developmental timeline of Cdh13 mRNA expression for whole brain (black bars) and hippocampus (gray bars; mean±s.e.m.; n=2 for hippocampus E18.5, P7, P21 and adult, for all other time points n=3). (b) Immunofluorescence staining of cultured hippocampal neurons for CDH13 (green) and the synaptic markers VGLUT, VGAT, PSD95 or Gephyrin (red). CDH13-positive puncta are indicated by the triangles, colored yellow for colocalized foci (VGAT) and blue for juxtaposed foci (Gephyrin). CDH13 localizes at the presynaptic side of inhibitory synapses. Scale bar, 1 μm. (c) Immunofluorescence staining of cultured hippocampal neurons for CDH13 (green) and GABA-A receptor α1 subunit (GABA-Aα1, red). CDH13-positive puncta are indicated by the triangles, colored blue for juxtaposition with GABA-Aα1-positive puncta. White arrows indicate puncta that are CDH13-negative and GABA-Aα1-positive. Scale bar, 1 μm. mRNA, messenger RNA; VGAT, vesicular GABA transporter; VGLUT, vesicular glutamate transporter.
