Figure 1 | Translational Psychiatry

Figure 1

From: A requirement of serotonergic p38α mitogen-activated protein kinase for peripheral immune system activation of CNS serotonin uptake and serotonin-linked behaviors

Figure 1

Activation and elimination of p38 MAPK in dorsal raphe (DR) 5-hydroxytryptamine (5-HT) neurons. Images of 5-HT and p38 MAPK staining for lipopolysaccharide (LPS) studies were collected from the dorsomedial division of the DR nucleus55 from adult male C57BL/6J mice. Images of the conditional p38α MAPK elimination experiments were obtained in the same manner. (a) Peripheral administration of LPS (0.2 mg kg−1 1 h before being killed) elevates levels of phospho-p38 MAPK immunoreactivity in 5-HT-labeled neurons of the adult mouse dorsomedial division of the DR. Scale bar, 10 μm. (b) Conditional elimination of p38α MAPK immunoreactivity. Immunofluorescence for p38α MAPK is presented for floxed p38α MAPK mice without (p38α5HT−) or with (p38α5HT+) expression Cre recombinase in 5-HT neurons via ePET::Cre as described in Materials and Methods. Scale bar, 5 μm.

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