Figure 1
From: Fingolimod induces neurogenesis in adult mouse hippocampus and improves contextual fear memory
(a) Illustrative graph that shows the exact time of administration of BrdU and fingolimod in animals for the NSCs proliferation experiments. (b) Coronal section of dorsal DG from 3-month-old C57/BL6 mice injected with fingolimod or vehicle daily for 14 days, co-injected with BrdU for the last 5 days. Sections were immunostained for BrdU (green) and counterstained with DAPI. (c) Quantification of BrdU+ cells in SGZ in Fingolimod or vehicle-treated mice of different ages. (d) Coronal section of dorsal DG from 3-month-old C57/BL6 mice injected with Fingolimod or vehicle daily for 14 days. Image depicts DcX (red) immunostained immature neurons. (e) Quantification of DcX cells in C57/bl6 mice of different ages treated with fingolimod or vehicle for 14 days. Scale bar, 150 μm (mean±s.d.; n=6; *P<0.05 vs vehicle). BrdU, 5-bromo-2′-deoxyuridine; DAPI, 4′,6-diamidino-2-phenylindole; DcX, doublecortin; DG, dentate gyrus; NSC, neural stem cells; SGZ, subgranular zone.
