Figure 4
Basal synaptic transmission in the LA is normal in Rapgef6 knockout mice. (a) Synaptic input–output curves for the EPSCs recorded at the cortico-LA synapses in slices from WT and HOM mice. The EPSCs were recorded under voltage-clamp conditions at a holding potential of −70 mV. (b) Same as in a, but the EPSCs were recorded in thalamic input to the LA. (c) Top, examples of paired-pulse facilitation of the cortico–amygdala EPSCs recorded at different interstimulus intervals (50 ms, 70 ms, 100 ms and 200 ms) at a holding potential of −70 mV in slices from WT and HOM mice. Superimposed traces are averages of 10 EPSCs at each interstimulus interval. Bottom, summary plot of paired-pulse facilitation experiments in cortico-LA projections. (d) The experiments were identical to c but the EPSCs were recorded in thalamic input to the LA. (e) Rapgef6 ablation had no effect on the parameters of glutamatergic mEPSCs. mEPScs were recorded in LA neurons at −70 mV in slices from WT (upper) and HOM mice (lower) in the presence of 1 μM TTX. (f) Summary plot showing mean peak amplitude (upper) and frequency (lower) of mEPSCs recorded in LA neurons in slices from WT and HOM mice. Results are shown as mean±s.e.m. EPSC, excitatory postsynaptic current; HET, heterozygous; HOM, homozygous; LA, lateral nucleus of the amygdala; TTX, tetrodotoxin; WT, wild type.
