Figure 3
From: Functional evaluation of a PTSD-associated genetic variant: estradiol regulation and ADCYAP1R1
(a) Using chromatin immunoprecipitation (ChIP) followed by quantitative PCR (qPCR), we measured binding of estrogen receptor alpha (ERα) to two regions of the genome: an estrogen response element (ERE), which contains rs2267735 in an intron of ADCYAP1R1 (ERE region), and a transcriptionally inactive region on chromosome 4 (negative control region). N=6 for each group. The qPCR measures obtained from the immunoprecipitated chromatin were divided by the measure obtained from the non-immunoprecipitated input sample (the amount of chromatin used in the ChIP experiment) using the same primers. The data represent the average percent of input ±s.d. for the two regions. (b) A competitive enzyme-linked immunosorbent assay was used to measure the binding of ERα to double-stranded DNA sequences (oligos) relative to the canonical ERE-binding sequence. The fluorescent measures obtained for the competing oligos were transformed by dividing these values by the fluorescent measure obtained for the canonical oligo (positive control). The data represent the averaged inverse of these values±s.d. for each experimental oligo (C allele and G alelle) and the non-canonical (negative control).
