Figure 2 | Translational Psychiatry

Figure 2

From: Glucagon-like peptide 1 receptor activation regulates cocaine actions and dopamine homeostasis in the lateral septum by decreasing arachidonic acid levels

Figure 2

Local activation of the glucagon-like peptide 1 receptor (GLP-1R) promotes septal dopamine transporter (DAT) membrane expression and [3H]DA uptake. (a) Representative immunoblot of surface and total DAT following slice treatment with vehicle (artificial cerebrospinal fluid, aCSF) or 1 nm GLP-1. (b) Ratio of DAT surface expression in GLP-1-treated ex vivo septal slices as percentage of average vehicle values (t(3)=10.12; **P<0.01 by Student’s t-test; n=4). The slices were treated with 1 nm GLP-1 for 20 min. (c) [3H]DA uptake in the LS from ex vivo slice preparation following 20 min treatment with vehicle (aCSF) or 10 nm GLP-1. Uptake was significantly elevated (t(4)=2.35; *P<0.05 by Student’s t-test; n=5). (d) Pretreatment with 100 nm Ex-9 for 10 min blocks the increase in [3H]DA uptake following treatment with 10 nm GLP-1 (t(3)=1.047; P=0.19 by Student’s t-test; n=4).

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