Figure 4

DNA methylation analyses for the Rxra and Ppara promotors and analyses of genes for DNA methyltransferase. Methylation levels of individual CpG sites for the Rxra a, b and Ppara promotors c, d in the cortex of the AA⁽⁺⁾/DHA⁽⁺⁾and AA⁽⁻⁾/DHA⁽⁻⁾ groups are shown. (e) Quantitative RT-PCR analysis of Dnmt1, Dnmt3a-1, Dnmt3a-2 and Dnmt3b. Gapdh was used as an internal control. P-values were calculated using two-tailed Mann–Whitney U tests. ^#P<0.1, *P<0.05, **P<0.01.