Figure 2
From: Nectin-3 modulates the structural plasticity of dentate granule cells and long-term memory
Effects of nectin-3 knockdown on the proliferation, differentiation and maturation of adult-born DG granule cells. (a) The volume of dorsal DG GCL was comparable between groups. (b) Nectin-3 knockdown did not alter the density of cells immunoreactive for MCM2, a marker of cell proliferation. Inserts show magnified images of MCM2-positive proliferating cells. (c) Nectin-3 knockdown did not significantly change the average density of cells immunoreactive for doublecortin, a marker of cell differentiation, but increased the number of category B and categories A+B doublecortin-positive cells. Insets show magnified images of doublecortin-positive cells of different morphology. (d) Nectin-3 knockdown increased the density of immature granule cells immunoreactive for calretinin. Insets show magnified images of calretinin-positive immature DG neurons. (e) Nectin-3 knockdown significantly reduced the levels of calbindin, a marker of mature granule cells, in all DG subregions. Insets show magnified images of calbindin-positive mature DG neurons. All scale bars=100 μm. *P<0.05. CT, control; DG, dentate gyrus; GCL, granule cell layer; KD, nectin-3 knockdown; MCM2, minichromosome maintenance complex component 2.
