Figure 3

JQ1-induced gene expression in WT and APP mice. (a) Upper panel: number of up- and downregulated genes affected by JQ1 treatment in WT and APP mice. Lower panel: Venn diagram showing limited overlap of genes affected in WT and in APP mice. (b) Heatmap of differentially expressed genes in APP-vehicle vs APP-JQ1 animals showed two main clusters of up- and downregulated genes. (c) qPCR was performed for selected genes affected by JQ1 treatment in WT mice. Correlation analysis shows that qPCR data were able to confirm RNA-seq findings. (d) qRT PCR was performed for selected genes affected by JQ1 treatment in APP mice. Correlation analysis shows that qPCR data were able to confirm RNA-seq findings. (e) Network of functional categories represented by JQ1-associated changes in APP animals based on the Biological Process ontology. The size of the nodes correlates inversely with statistical significance. Categories of upregulated genes are red, downregulated genes are blue and those where there are both up- and downregulation are purple.